![]() ![]() ![]() Instructions, DNA samples for electrophoresis, practice gel loading solution, UltraSpec-Agarose™, electrophoresis buffer, pipets, 5 pre-cut nylon membranes, 5 pre-cut blotting filter papers, Blue-Blot DNA Stain™. easy to read chapter 16: hybridization, southern blot, northern blot, and microarrays dna hybridization and probes some of the first techniques developed. Southern blotting is a detection technique used to find the target DNA sequences in the DNA sample in the field of molecular biology. Our modified southern blot procedure is fast, safe, and designed for the classroom.Analyze DNA RFLP patterns from mother, child, and potential fathers to resolve the case Southern blotting is a hybridization technique for identification of particular size of DNA from the mixture of other similar molecules.Visualize the results using a modified Southern blot Isolate the DNA in question from the rest of the cellular material in the nucleus.Run electrophoresis on four DNA samples pre-digested with a restriction enzyme.Explore genetics and Southern blotting while solving a paternity case.DNA fragments are first separated by agarose gel electrophoresis, then transferred to a nylon membrane and finally visualized by staining. This experiment introduces your students to Southern blotting as a tool for "DNA Fingerprinting" in a hypothetical paternity determination.
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